Description
Aviva Systems Biology PLBD2 ELISA Kit (Human) (OKEH06535) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-PLBD2 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated PLBD2 and incubated. The PLBD2 found in the sample or standards competes with the biotinylated PLBD2 for limited binding sites on the immobilized anti-PLBD2 antibody. Excess unbound biotinylated PLBD2 and sample or standard PLBD2 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated PLBD2 captured in the well and inversely proportional to the amount of PLBD2 which was contained in the sample or standard